Identification and expression analysis of heat‐shock proteins in wheat infected with powdery mildew and stripe rust

Heat-shock proteins (HSPs), which are encoded by conserved gene families in plants, crucial for development and responses to diverse stresses. However, the wheat (Triticum aestivum L.) HSPs have not been systematically classified, especially those involved protecting plants from disease. Here, we classified 119 DnaJ (Hsp40) (TaDnaJs; 313 genes) 41 Hsp70 (TaHsp70s; 95 into six four groups, respectively, via a phylogenetic analysis. An examination of protein sequence alignment revealed diversity TaDnaJ structural organization, but highly J-domain, was usually characterized an HPD motif followed DRD or DED motifs. The expression profiles HSP-encoding homologous genes varied response Blumeria graminis f. sp. tritici (Bgt) Puccinia striiformis (Pst) stress. A quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated lack similarity DnaJ70b, Hsp70-30b, Hsp90-4b Bgt-infected resistant susceptible wheat. Furthermore, direct interaction between DnaJ70 TaHsp70-30 detected yeast two-hybrid (Y2H) assay, screening cDNA library Y2H evidence supported that only interacts directly with heat-shock transcription factor (HSF) A9-like also TaHsp90-4 HSP organizing protein. This study structure wheat, may be useful future functional elucidation fungal infections.